Equipment

10x Genomics’ Chromium platform is powered by Next GEM (Gel Bead-in-emulsion) technology. The key to this technology is the ability to generate tens of thousands of single cell partitions, each containing an identifying barcode for downstream analysis. It can perform single cell partitioning and barcoding in a matter of minutes. 

The ME220 focused-ultrasonicator is optimized for 1 to 8 sample batch processing for mechanical shearing for next-generation sequencing, NGS-grade nucleic acid extraction from FFPE tissue, whole blood, and dried blood spots, and biomarker extraction using real-time monitoring and integrated quality control with SonoLab software.

The NanoDrop 3300 significantly lowers the mass detection limit more than an order of magnitude, compared to conventional fluorometers for the quantification of DNA, RNA, and protein.

The Qubit 3.0 is more sensitive than UV absorbance–based quantification because the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target. Highly accurate quantification of DNA, RNA, and protein is performed rapidly in less than 3 seconds per sample.

Provides fully automated electrophoretic sample processing, for quick and reliable DNA and RNA quality control within any next-generation sequencing, microarray or quantitative PCR workflow. DNA integrity number (DIN) and RNA integrity number equivalent (RINe) deliver an objective evaluation of DNA or RNA degradation.

With automated electrophoresis, the Agilent 2100 Bioanalyzer system provides sizing, quantitation, and purity assessments for DNA, RNA, and protein samples. The bioanalyzer is ideal for fast and high resolution analysis of fragmented DNA, DNA libraries, total RNA, mRNA, and miRNA for sensitive downstream applications.

A high-performance, high-throughput real-time PCR platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation.

The NextSeq 2000 uses 2-channel chemistry and patterned flow cells to increase the output. The NextSeq 2000 is highly flexible and supports a broad range of next generation sequencing applications, including single-cell gene expression, whole genome sequencing, shotgun metagenomics, RNA sequencing, and infectious disease research. It increases the throughput and decreases the costs per run compared to its predecessor, the NextSeq 500.